Reengineering Inducible Cardiac-Specific Transgenesis With an Attenuated Myosin Heavy Chain Promoter
نویسندگان
چکیده
منابع مشابه
Reengineering inducible cardiac-specific transgenesis with an attenuated myosin heavy chain promoter.
Despite the advantages of reversibly altering cardiac transgene expression, the number of successful studies with inducible cardiac-specific transgene expression remains limited. The utility of the current system is hampered by the large number of lines needed before a nonleaky inducible line is isolated and by the use of a heterologous virus-based minimal promoter in the responder line. We dev...
متن کاملRole of USF1 phosphorylation on cardiac -myosin heavy chain promoter activity
Xiao, Qianxun, Agnes Kenessey, and Kaie Ojamaa. Role of USF1 phosphorylation on cardiac -myosin heavy chain promoter activity. Am J Physiol Heart Circ Physiol 283: H213–H219, 2002. First published March 28, 2002; 10.1152/ajpheart.01085.2001.—Contractile activity of the cardiac myocyte is required for maintaining cell mass and phenotype, including expression of the cardiac-specific -myosin heavy...
متن کاملCoexpression of Myosin Heavy Chain 2b with Myosin Heavy Chain 1- Fact or Artefact
In skeletal muscle, pure fibres expressing one myosin heavy chain (MyHC) isoform, and intermediate fibres, expressing two and exceptionally three MyHCs have been described. When skeletal muscle adapts its fibre type profile to changed functional demands MyHC isoform transformation follows the pathway: MyHC-1 ↔ MyHC-2a ↔ MyHC-2x/d ↔ MyHC-2b. Therefore, in hybrid fibres only successive isoforms f...
متن کاملAn a-cardiac myosin heavy chain gene mutation impairs contraction and relaxation function of cardiac myocytes
SONG-JUNG KIM,1 KENJI IIZUKA,1 RALPH A. KELLY,2 YONG-JIAN GENG,1 SANFORD P. BISHOP,1 GUIPING YANG,1 AMELIA KUDEJ,1 BRADLEY K. MCCONNELL,3 CHRISTINE E. SEIDMAN,4 JONATHAN G. SEIDMAN,3 AND STEPHEN F. VATNER1 1Cardiovascular and Pulmonary Research Institute, Allegheny University of the Health Sciences, Pittsburgh, Pennsylvania 15212; 3Department of Genetics, Harvard Medical School and Howard Hughe...
متن کاملAn α-cardiac myosin heavy chain gene mutation impairs contraction and relaxation function of cardiac myocytes.
Left Ventricular (LV) myocytes were isolated from 15-wk-old male mice bearing the Arg403 → Gln α-cardiac myosin heavy chain missense mutation (α-MHC403/+), a model of familial hypertrophic cardiomyopathy. LV myocytes were classified morphologically: type I, rod shaped with parallel myofibrils; type II, irregularly shaped, shorter and wider than wild-type (WT) control cells, with parallel myofib...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Circulation Research
سال: 2003
ISSN: 0009-7330,1524-4571
DOI: 10.1161/01.res.0000065442.64694.9f